RNA Sequencing

PacBio long-read sequencing offers enhanced RNA sequencing capabilities with isoform sequencing, which produces full-length transcripts without the need for assembly.

Produce full-length transcripts without assembly

The isoform sequencing (Iso-Seq) application generates full-length cDNA sequences — from the 5’ end of transcripts to the poly-A tail — eliminating the need for transcriptome reconstruction using isoform-inference algorithms. The Iso-Seq method generates accurate information about alternatively spliced exons and transcriptional start sites. It also delivers information about poly-adenylation sites for transcripts up to 10 kb in length across the full complement of isoforms within targeted genes or the entire transcriptome.

 

PacBio delivers confident characterization of isoform diversity with the Sequel System

  • Profile the full complexity of the human transcriptome and discover novel genes, isoforms, and gene fusion events
  • Survey transcript diversity in plants and animals to improve genome annotation and gene discovery
  • Improve quantitation accuracy of short-read RNA-seq data with sample-specific gene isoform models
  • Sequence full-length bacterial operons from transcriptomes and metatranscriptomes

Application Brief Long-read RNA sequencing – Best Practices

To learn more about how to profile the complexity of the transcriptome with the Iso-Seq application, contact us.