Amplicon sequencing has emerged as a reliable and cost-effective ultra-deep targeted, next- generation sequencing (NGS) method to discover and profile known, rare, and novel variants.
Bisulfite sequencing permits analysis of epigenetic status at a single nucleotide resolution. Through bisulfite or oxidative bisulfite sequencing, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) can be identified in the genome, respectively.
ChIP-seq has emerged as the favored method for producing genome-wide epigenetic profiles for chromatin proteins and transcription factors.
Circulating, Cell-Free DNA
Circulating, cell-free nucleic acids have emerged as important bio markers in cancer and clinical diagnostics and detection of various clinical conditions.
Formalin-fixed, paraffin-embedding (FFPE) is a standard method for long term preservation of millions of archived human tissue samples. Swift Biosciences offers sample preparation solutions to overcome these limitations by assessing low frequency somatic mutations that are key to cancer progression, offering desirable biomarkers for ultimately improving clinical outcomes for a wide spectrum of oncology-related diseases.
Hybridization Capture Sequencing
Swift Biosciences offers a solution to construct bias-free libraries for target enrichment utilizing hybridization-based capture technologies.
The Accel-NGS® 2S MID Indexing Kits have been designed, optimized, and validated for use with Accel-NGS 2S DNA Library Kits on Illumina® platforms, and aid in low frequency variant detection, as well as accurate de-duplication of single read sequencing and sequencing from samples with non-random fragmentation.
Bias introduced by amplification strategies and “hard-to-crack” microbes within a population remains a challenge in metagenomics analyses. The Accel-NGS® 1S Plus and 2S DNA Library Preparation Kits allow users to enhance the accuracy of their metagenomics analyses using strategies that avoid amplification-introduced bias and enhance recovery of hard to extract microbial DNA.
Single-Cell Methylation Sequencing
Epigenetic markings, such as DNA methylation, can be used to identify cell types and the regulatory elements underlying cell-specific function. This high-throughput single-cell sequencing approach can be used for many applications, such as assessing normal tissue for regulation of cell differentiation, disease state to gain insight on epigenomics alterations, and cross-species to identify evolutionary conservation of epigenomics regulation.
Download: Single-Cell Methylation Sequencing
Whole Genome Sequencing
Accel-NGS® 2S DNA Library Kits can help you maximize your data out put, giving you the best genome-wide coverage of any kit on the market, and reducing your sequencing costs.